Breakthrough in Industry-University-Research Collaborative Innovation! Focusfreda and Jiangnan University Joint Research Achievement Featured on Cover of Top Synthetic Biology Journal
Amidst the rapid development of green bio-manufacturing technologies, industrial biotechnology is driving industrial upgrades in enzyme preparation, functional chemicals, and related fields through the optimization of microbial chassis cells. Recently, the collaborative research achievement titled "Unlocking Green Bio-Manufacturing Potential: Superior Heterologous Gene Expression with a T7 Integration Overexpression System in Bacillus subtilis" by Shandong Focusfreda Biotech Co., Ltd. and the System Fermentation and Pharmaceutical Engineering Laboratory of Jiangnan University was featured as the cover paper in the international authoritative synthetic biology journal ACS Synthetic Biology (Biology Q2), marking a breakthrough in the industry-university-research collaboration system established by Focusfreda and Jiangnan University.
(ACS Synthetic Biology Journal Cover)
The research team innovatively established a dual-module T7 integration expression system in Bacillus subtilis. The first module integrates T7 RNA polymerase into the genome under the control of the P43 promoter. The second module, responsible for expression regulation, was integrated into 28 distinct genomic loci for characterization. Using green fluorescent protein (GFP) as a reporter gene, the team systematically explored the impact of genomic positions on gene expression, ultimately obtaining a high-strength integrated expression system. Additionally, by modifying the LacI repressor for biocontrol, high expression strength was achieved without inducer addition, and the stability of the system was evaluated. This system successfully expressed phospholipase D and hyaluronate lyase, achieving extracellular enzyme activities of 339.12 U/mL and 2.60×10⁴ U/mL, respectively. Furthermore, the HA gene cluster was expressed to validate the system’s capability for long gene cluster expression, achieving an HA production yield of 6.86 g/L at a 5 L fermentation scale. This system eliminates the need for antibiotics and inducers, providing Bacillus subtilis with a controllable, efficient, and promising gene expression regulation tool, significantly enhancing its potential in bio-manufacturing applications.
The deep collaboration between Focusfreda and Jiangnan University fully integrates the foundational research strengths of academia with industrial expertise. This publication in a top synthetic biology journal signifies a critical breakthrough in microbial chassis cell engineering. Supported by projects such as the National Natural Science Foundation of China, the Central University Basic Research Fund, and the Taishan Industrial Leading Talent Program, this research highlights the remarkable effectiveness of the "industry-university-research-application" collaborative innovation model. The developed system not only provides novel tools for metabolic engineering of Bacillus subtilis but also opens sustainable pathways for green manufacturing of functional nutritional chemicals and industrial enzymes, holding strategic significance for advancing bio-manufacturing industry upgrades.
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